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These risk factors include: [22] Sexual activity Strenuous physical activity, such as frequent bicycle or motorcycle riding Prolonged sitting, such as frequent travel or truck driving History of prostate or urinary tract infections Benign enlarged prostate or prostate surgery, typical in older men Anatomic defects such as a posterior urethral meatus, which occur in prepubescent boys Testicular trauma is caused by a twisted testicle not getting enough blood

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After homogenization, the samples were centrifuged at 2,000 g to remove large debris, and the supernatants were filtered in Spin-X 0.45 m pore filters (Costar, NY) at 10,000 g for 10 to 20 min