Single-cell suspensions were diluted, centrifuged, washed once with PBS/DTT and twice with PBS, before cryopreservation (F-media, 40% FBS, 10% DMSO)
Cake is very difficult to break up
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To view a copy of this license, visit About this article Cite this article Alqahtani, J.S., Aldhahir, A.M., Oyelade, T