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Cysteine-rich protein 61 (CCN1) domain-specific stimulation of matrix metalloproteinase-1 expression through V3 integrin in human skin fibroblasts

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On day 7, supernatant was collected, and proteins were purified through affinity (Protein G Resin, Genscript or Ni-IMAC Resin, ThermoFisher) and size-exclusion chromatography (Cytiva) sequentially

Withdraw 0.5ml of water per mg of peptide powder, then proceed to pierce the peptide vial and allow the water to gently trickle down the side of the vial and naturally meet with the powder, thus melting it

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