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After incubation, and washes with PBS, appropriate secondary fluorescent antibodies (1:500, AlexaFluor-conjugated, Invitrogen) were added to the blocking solution at room temperature for 2 h in the dark

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After being washed in PBS, the slides were stained with hematoxylin and eosin, mounted with Canada balsam (03984, Sigma-Aldrich), and observed with an epifluorescence microscope (IX71, Olympus, Tokyo, Japan) equipped with a digital imaging system (DP71, Olympus)

Patients should be instructed to seek immediate medical care if they experience severe, persistent abdominal pain that may radiate to the back

Supporting Liver Health During Weight Loss As you lose weight, your liver has to work overtime