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The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

Grigolo B, Roseti L, Fiorini M, Facchini A

Some mucin genes, however, were expressed only in ASG-10, including one mucin-2-like, a mucin 5AC like and a mucin 13-like

adult Tolerable Upper Intake Level for supplemental alpha-tocopherol is 1,000 mg per day, but lower doses may still be inappropriate for some users

Methods Chondrocytes were treated with H 2 O 2 for 1, 4, and 16 h followed by protein extraction and processing, including denaturation, alkylation, and trypsin digestion