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Studies show that direct injection onto lyophilised powder reduces bioactivity by 1218% compared to wall-injection methods, and vigorous shaking causes 2535% degradation even when the solution appears visually clear

For context, gastric bypass surgery typically produces 2535% weight loss over a comparable timeframe

We expressed this in a eukaryotic expression system because this is the only established system that is suitable for the expression of complex recombinant proteins with human-like glycoforms that are bioactive in humans (Maccani et al

10.3389/fonc.2021.698023 79 LiuS

Pasini E, Aquilani R, Testa C, Baiardi P, Angioletti S, et al