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#205311, Qiagen), following the manufacturers instructions

Defense-related genes encoding osmotin, PR-1b, chitinase, and superoxide dismutase (SOD) enzymes were up-regulated in the transgenic lines as compared to wild-type plants

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Nrf2 functions by blocking GSH depletion-mediated lipid peroxidation in HCC cells and plays a central role in protecting them from sorafenib-induced ferroptosis (Sun et al., 2016b)