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Documented prior attempts at lifestyle modification

GSH and GSSG determination To assess the influence of FLC and MCFG on glutathione intracellular contents, each strain was cultured in YNB medium at 28C, for 24 h, using an inoculum size of approximately 10 7 cells/ml to recover adequate amount of cytosolic material for glutathione analysis

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Lifestyle factors deserve final assessment before escalating pharmacotherapy