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To preclude reoxygenation, all cells designated for Western blot analysis or RNA extraction were subjected to three sequential washes with PBS within the hypoxic workstation, followed immediately by lysis using the appropriate buffer (RIPA lysis or Trizol reagent)

The obtained spectra were background-corrected

A recent study demonstrated the ability of a dendrimer-conjugated NAC (D-NAC) to specifically target glial and astrocyte cells, indicating potential to alter GSH levels in specific cell populations without altering levels throughout the CNS (Nance et al., 2017

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