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After 8+ hours at room temperature, discard the peptide entirely

A Shimadzu LC20AD, Ultra Performance Liquid Chromatography (UPLC) system (Shimadzu Corporation, Kyoto, Japan) equipped with Shimadzu 8040 triple quadrupole (QqQ) mass analyzer (Shimadzu Corporation, Kyoto, Japan) with an electrospray ionization source (ESI) operated in the positive charge mode for the quantification of 2

Acetyl-CoA synthetase (ACSS2) does not generate butyryl- and crotonyl-CoA

one that's too acidic may sting or cause sensitivity

In mammalian cells, the receptor function of Na,K-ATPase activated by CTSs also include Src-independent pathways