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Opening of NMDA channels (which relates to the level of cellular depolarization) leads to a rise in post-synaptic Ca 2+ concentration and this has been linked to long-term potentiation, LTP (as well as to protein kinase activation)

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When cells grew to 90% confluence, they were starved for 24 h in serum-free medium

Wolfram-Schauerte, M

Preparation and Cytotoxicitiy Tests of of PCG-MSNs Assembled H1, H2 and H3 Probes PCGs-MSNs were dispersed in 2 mL hybridization buffer (pH 7.4) and stirred continuously at 37 C for 90 min to obtain the PCG-MSNs solution (10 mg/ml)