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After adding 340 L 4 C cold mixture of methanol and acetonitrile (ACN) (25:75, v/v) and 50 L of a 2 M D 9 -trimethylamine-N-oxide (Cambridge Isotopes Laboratories, Tewksbury, MA, USA) solution in methanol, the protein was precipitated by vortex-mixing for 30 s followed by 10 min incubation

The GLP-1RA exenatide was shown to significantly lower BMI and 2-hour glucose in adolescents with obesity, in the placebo-controlled, randomized controlled trial Combat-JUDO

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evidence suggests that IL-1 can accelerate anabolic activities in chondrocytes 15

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