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Activity of caspase-8 determines plasticity between cell death pathways

Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

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Knockout of HGMB1 promoted the expression of NRF2 and its downstream targets, including HO-1, NQO1, GCLC, and GCLM, prevented the production of ROS and LDH and upregulated the level of GPX4 in renal mesangial cells (Fig