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Nat Rev Immunol 16:639649

falciparum -infected erythrocytes (trophozoite stage 2630 h post invasion) were washed three times with pre-warmed (37C) Ringer's solution (122.5 mM NaCl, 5.4 mM KCl, 1.2 mM CaCl 2 , 0.8 mM MgCl 2 , 11 mM D-glucose, 25 mM Hepes, 1 mM NaH 2 PO 4 , pH 7.4) and seeded in 50 l Ringer's solution on poly-L-lysin-coated -slides VI (Ibidi, Martinsried, Germany)

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& Hara, T

The water should run down the glass and pool at the bottom, gradually surrounding the lyophilised peptide cake