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Cytokines Measurement The levels of TNF- and IL-1 in serum and peritoneal lavage fluid collected at 24 h post CLP induction were quantified by Enzime-Linked Immunosorbent Assay (ELISA) using an appropriate commercial kit (R&D Systems, Minneapolis, Canada) according to the manufacturer's instructions

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Microdosing decisions in the Calibrate phase should therefore take systemic hormone status into account, not simply whether vaginal symptoms have improved

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